UJI FITOKIMIA EKSTRAK METANOL KULIT BATANG KETEPENG CINA (Cassia alata L.) SEBAGAI KANDIDAT OBAT TRADISIONAL
DOI:
https://doi.org/10.26418/edunaturalia.v6i1.92642Abstract
This study aims to identify the content of phytochemical compounds in the methanol extract of the bark of Ketepeng Cina (Cassia alata L.) as an initial step in the development of traditional medicine. The Cassia alata plant is known to have various potential pharmacological properties. This study used a qualitative descriptive research design to determine the results of the phytochemical test. This test was conducted at the Research Laboratory of the Department of Chemistry, Faculty of Mathematics and Natural Sciences, Syiah Kuala University. Extraction was carried out using methanol solvents through the maceration method, then continued with phytochemical tests to detect the presence of secondary metabolite compounds such as alkaloids, flavonoids, saponins, tannins, steroids, and triterpenoids. The test results showed that the methanol extract of the bark of Cassia alata contains several active compounds including steroids, flavonoids, phenolics and tannins which have the potential as raw materials for traditional medicine. These findings support the use of this plant in the development of phytopharmaceuticals and encourage further research for pharmacology and toxicity tests. Keywords: Cassia alata L., Phytochemicals, Methanol extract, Traditional medicineReferences
Besharati, M., Maggiolino, A., Palangi, V., Kaya, A., Jabbar, M., Eseceli, H., De Palo, P., & Lorenzo, J. (2022). Tannin in Ruminant Nutrition: Review. Molecules, 27. https://doi.org/10.3390/molecules27238273.
Bhambhani, S., Kondhare, K., & Giri, A. (2021). Diversity in Chemical Structures and Biological Properties of Plant Alkaloids. Molecules, 26. https://doi.org/10.3390/molecules26113374.
Chew, Y., Khor, M., Xu, Z., Lee, S., Keng, J., Sang, S., Akowuah, G., Goh, K., Liew, K., & Ming, L. (2022). Cassia alata, Coriandrum sativum, Curcuma longa and Azadirachta indica: Food Ingredients as Complementary and Alternative Therapies for Atopic Dermatitis-A https://doi.org/10.3390/molecules27175475. Comprehensive Review. Molecules, 27.
Chung, K., Wong, T., Wei, C., Huang, Y., & Lin, Y. (1998). Tannins and Human Health: a review. Food Science and Nutrition, 38 6, 421-64. https://doi.org/10.1080/10408699891274273.
De Boer, H., & Cotingting, C. (2014). Medicinal Plants for Women's Healthcare in Southeast Asia: A Meta-Analysis of Their Traditional Use, Chemical Constituents, and Pharmacology. Journal of Ethnopharmacology, 151(2), 747–767. https://doi.org/10.1016/j.jep.2013.11.030
Debnath, B., Singh, W., Das, M., Goswami, S., Singh, M., Maiti, D., & Manna, K. (2018). Role of Plant Alkaloids on Human Health: A Review of Biological Activities. Materials Today Chemistry, 9, 56–72. https://doi.org/10.1016/j.mtchem.2018.05.001
Diliarosta, S., Ramadhani, R., & Indriani, D. (2020). Diversity of Pteridophyta in Lubuak Mato Kuciang Padang Panjang, Sumatera Barat. Polymer Journal, 12, 180-185. https://doi.org/10.5530/pj.2020.12.27.
Egra, S., Mardiana, M., Kurnia, A., Kartina, K., Murtilaksono, A., & Kuspradini, H. (2019). Uji Potensi Ekstrak Daun Tanaman Ketepeng (Cassia Alata L.) dalam Menghambat Pertumbuhan Bakteri Ralstonia solanacearum dan Streptococcus sobrinus. ULIN: Jurnal Hutan Tropis, 3(1), 25–31. https://doi.org/10.32522/ujht.v3i1.2059
Fatmawati, S., Yuliana, Purnomo, A. S., & Abu Bakar, M. F. (2020). Chemical Constituents, Usage and Pharmacological Activity of https://doi.org/10.1016/j.heliyon.2020.e04396 Cassia Alata. Heliyon, 6(7), e04396.
Hagerman, A. E., Riedl, K. M., Jones, G. A., Sovik, K. N., Ritchard, N. T., Hartzfeld, P. W., & Riechel, T. L. (1998). High Molecular Weight Plant Polyphenolics (Tannins) as Biological Antioxidants. Journal of Agricultural and 143 EduNaturalia: Jurnal Biologi dan Kependidikan Biologi Food Chemistry, 46(5), 1887–1892. https://doi.org/10.1021/jf970975b
Hamni, L., Avidlyandi, A., Banon, C., Wiradimafan, K., Triawan, D., Wibowo, R., & Adfa, M. (2022). Kombinasi Ekstrak Metanol Daun Ketepeng Cina (Cassia Alata Linn.) dengan Enam Ekstrak Tumbuhan Terpilih dalam Menghambat Pertumbuhan Bakteri Staphylococcus Aureus. BIOEDUSAINS: Jurnal Pendidikan Biologi Dan Sains, 5(2), 123–130. https://doi.org/10.31539/bioedusains.v5i2.3996
Hanifa, A. P., Erliana, D., Avidlyandi, A., Rizka, M., Triawan, D. A., Yudha, S. P., & Adfa, M. (2022). Efektivitas Ekstrak Metanol Daun Ketepeng Cina (Cassia Alata L.) dalam Menghambat Pertumbuhan Bakteri Staphylococcus Epidermidis Secara Tunggal dan Kombinasi. Jurnal Biosilampari: Jurnal Biologi, 5(1), 57–66. https://doi.org/10.31540/biosilampari.v5i1.1787
Hashim, S. E., Basar, N., Abd Samad, A., Jamil, S., Bakar, M. B., Jamalis, J., Abd-Aziz, N., Wagiran, A., Razak, M. N. A., & Abd Samad, A. (2024). Advancing Alkaloid-Based Medicines: Medical Applications, Scalable Production and Synthetic Innovations. Phytochemistry Reviews, 23(1), 1–29. https://doi.org/10.1007/s11101 024-10050-0.
Hoshino, Y., & Villanueva, L. (2023). Four Billion Years of Microbial Terpenome Evolution. FEMS Microbiology Reviews, 47(2). https://doi.org/10.1093/femsre/fuad008.
Li, S., Liu, F., Zhang, K., Tong, Y., & Liu, X. (2021). Research Progress on the Mechanism of Natural Product Ingredients in the Treatment of Uveitis. Journal of Immunology Research, 2021, Article ID 6683411. https://doi.org/10.1155/2021/6683411.
Masyita, R., Barokah, Z., Anggraeni, Y., Soleha, M., & Angelina, M. (2024). Effect of pH Variation on Chemical Components and Antifungal Activity of Ketepeng Cina (Cassia Alata L.) Leaves Extract. IOP Conference Series: Earth and Environmental Science, 1312(1), 012012. https://doi.org/10.1088/1755-1315/1312/1/012012
Mawaddah, I., Erwin, E., & Saleh, C. (2020). Skrining Fitokimia, Uji Toksisitas dan Uji Peredaman Radikal DPPH Ekstrak Daun Gelinggang (Cassia Alata L.). KOVALEN: Jurnal Riset Kimia, 6(1), 61–66. https://doi.org/10.22487/kovalen.2020.v6.i1.15045
Mi, L., Li, Y., Sun, M., Zhang, P., Li, Y., & Yang, H. (2021). A Systematic Review of Pharmacological Activities, Toxicological Mechanisms and Pharmacokinetic Studies on Aconitum Alkaloids.. Chinese Journal of Natural Medicines, 19 7, 505-520 . https://doi.org/10.1016/S1875-5364(21)60050-X.
Michalak, M. (2023). Plant Extracts as Skin Care and Therapeutic Agents. International Journal of Molecular Sciences, 24(20), 15444. https://doi.org/10.3390/ijms242015444
Mou, Z., Zhao, Y., Ye, F., Shi, Y., Kennelly, E., Chen, S., & Zhao, D. (2021). Identification, Biological Activities and Biosynthetic Pathway of Dendrobium Alkaloids. Frontiers in Pharmacology, 12, 605994. https://doi.org/10.3389/fphar.2021.605994
Ntandou, G. F., Nsonde, J. C., Boungou, M., Banzouzi, J. T., & Banzouzi, G. (2020). Anticonvulsant and Sedative Effects of Cassia Alata (Fabaceae) in Mice. Global Journal of Health Science Research, 5(1), 28–37.
Nuryati, N., Amalia, R. R., Hairiyah, N., & Nurisyah, N. (2023). Optimasi Metode Ekstraksi Terhadap Parameter Mutu Ekstrak Daun Ketepeng Cina (Senna Alata). Jurnal Teknologi Agro-Industri, 10(2), 183–190. https://doi.org/10.34128/jtai.v10i02.183
Okuda, T., & Ito, H. (2011). Tannins of Constant Structure in Medicinal and Food Plants—Hydrolyzable Tannins and Polyphenols Related to Tannins. Molecules, 16, 2191 - 2217. https://doi.org/10.3390/molecules16032191.
Osbourn, A. E. (2003). Saponins in Cereals. Phytochemistry, 62(1), 1–4. https://doi.org/10.1016/S0031 9422(02)00393-X
PatrÃcio, K., Minato, A., Brolio, A., Lopes, M., Barros, G., Moraes, V., & Barbosa, G. (2022). Medicinal Plant Use in Primary Health Care: An Integrative Review. Ciencia & Saude Coletiva, 27(2), 677–686. https://doi.org/10.1590/1413-81232022272.46312020
Prasathkumar, M., Anisha, S., Dhrisya, C., Becky, R., & Sadhasivam, S. (2021). Therapeutic and Pharmacological Efficacy of Selective Indian Medicinal Plants – A Review. Phytomedicine Plus, 1(2), 100029. https://doi.org/10.1016/J.PHYPLU.2021.100029
Pucker, B., & Selmar, D. (2022). Biochemistry and Molecular Basis of Intracellular Flavonoid Transport in Plants. Plants, 11. https://doi.org/10.3390/plants11070963.
R. B., Mani, R., B. A., C. P., Shankar, J., M. K., & C. M. (2022). Cassia Alata: Phytopharmacological, Traditional, and Medicinal Considerations. World Journal of Current Medical and Pharmaceutical Research, 4(6), 147–150. https://doi.org/10.37022/wjcmpr.v4i6.244.
Rahman, M., Rahaman, M., Islam, M., Rahman, F., Mithi, F., Alqahtani, T., Almikhlafi, M., Alghamdi, S., Alruwaili, A., Hossain, M., Ahmed, M., Das, R., Emran, T., & Uddin, M. (2021). Role of Phenolic Compounds in Human Disease: Current Knowledge and https://doi.org/10.3390/molecules27010233 Future Prospects. Molecules, 27(1), 233.
Saptarini, N., Mustarichie, R., Hasanuddin, S., & Corpuz, M. (2024). Cassia Alata L.: A Study of Antifungal Activity Against Malassezia Furfur, Identification of Major Compounds, And Molecular Docking to Lanosterol 14 Alpha Demethylase. Pharmaceuticals, 17(3), 380. https://doi.org/10.3390/ph17030380
Sauter, E. (2020). Cancer Prevention and Treatment Using Combination Therapy with Natural Compounds. Expert Review of Clinical Pharmacology, 13(3), 265–285. https://doi.org/10.1080/17512433.2020.1738218
Scholz, B., & Liebezeit, G. (2006). Chemical Screening for Bioactive Substances in Culture Media of Microalgae and Cyanobacteria from Marine and Brackish Water Habitats: First Results. Pharmaceutical Biology, 44, 544 - 549. https://doi.org/10.1080/13880200600883114.
Sikumbang, I., Saputro, A., Vischa, R., & Lutfiyati, H. (2019). Silat (Cassia Alata) Handwash Sebagai Pembasmi Bakteri Staphylococcus Aureus. Jurnal https://doi.org/10.31603/pharmacy.v5i1.2072 Farmasi Sains dan Praktis, 5(1), 14–19.
So, O., O, A., & Ka, J. (2018). Medicinal Plants and Sustainable Human Health: A Review. Horticulture International Journal, 2(2), 51. https://doi.org/10.15406/HIJ.2018.02.00051
Somchit, M., Reezal, I., Nur, I., & Mutalib, A. (2003). In Vitro Antimicrobial Activity of Ethanol and Water Extracts of Cassia Alata. Journal Of Ethnopharmacology, 84 1, 1-4. https://doi.org/10.1016/S0378-8741(02)00146-0.
Sun, J., Liu, B., Rustiami, H., Xiao, H., Shen, X., & K. (2024). Mapping Asia Plants: Plant Diversity and A Checklist of Vascular Plants in Indonesia. Plants, 13(16), 2281. https://doi.org/10.3390/plants13162281
Sun, W., & Shahrajabian, M. (2023). Therapeutic Potential of Phenolic Compounds in Medicinal Plants—Natural Health Products for Human Health. Molecules, 28(4), 1845. https://doi.org/10.3390/molecules28041845
Tcheghebe, O. T., Raïssa, V. S. T., & Tala, S. (2017). Ethnobotanical Uses, Phytochemical and Pharmacological Profiles, And Toxicity of Cassia alata L. The Pharma Innovation Journal, 6(6), 1–6. https://doi.org/10.22271/tpi.2017.v6.i6a.1075
Tetali, S. D. (2019). Terpenes and Isoprenoids: A Wealth of Compounds for Global Use. Planta, 249(1), 1–8. https://doi.org/10.1007/s00425-018-3056-x
Utari, D., & Indrayudha, P. (2019). Uji antibakteri 4 tanaman (Ketepeng Cina, Sambung Nyawa, Pegagan, dan Bayam Merah) terhadap Staphylococcus aureus ATCC 25923 Sensitif dan Resisten. Skripsi, Universitas Muhammadiyah Surakarta. http://eprints.ums.ac.id/75675/
Yang, P., Dai, J., Gu, X., Xiong, W., Huang, D., Qiu, S., Zheng, J., Li, Y., Yang, F., & Zhou, M. (2022). Indole Alkaloids and Chromones from The Stem Bark of Cassia Alata and Their Antiviral Activities. Molecules, 27(10), 3129. https://doi.org/10.3390/molecules27103129
Zhang, H., Wang, X., Guo, Y., Liu, X., Zhao, X., Teka, T., Lv, C., Han, L., Huang, Y., & Pan, G. (2020). Thirteen Bisbenzylisoquinoline Alkaloids in Five Chinese Medicinal Plants: Botany, Traditional Uses, 145 EduNaturalia: Jurnal Biologi dan Kependidikan Biologi Phytochemistry, Pharmacokinetic and Toxicity Studies. Journal of Ethnopharmacology, 259, 113566. https://doi.org/10.1016/j.jep.2020.113566
Zhou, M., Liu, C., Yang, J., Niu, D., Dong, M., Yang, G., Li, X., & Hu, Q. (2020). Alatains A and B, Unique Hetero Dimeric Polyphenols from Cassia alata and Their Anti-Tobacco Mosaic Virus Activity. Fitoterapia, 146, 104763. https://doi.org/10.1016/j.fitote.2020.104763
Zubaydah, W., Sahumena, M., Adawia, S., Wahyuni, R., Adjeng, A., Nisa, M., Kasmawati, H., Ihsan, S., R., & Aswan, M. (2019). Preparation and Characterization of Self-Nanoemulsifying Drug Delivery System (SNEDDS) from Moringa oleifera L. and Cassia alata L. Leaves Extracts. Proceedings of the 2nd International Conference on Bioscience, Biotechnology, and Biometrics 2019. https://doi.org/10.1063/1.5141325
Downloads
Published
Issue
Section
License
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution-ShareAlike License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are not restricted by the journal from entering into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., posting it to an institutional repository or publishing it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) after its publication in this journal. This approach aims to safeguard against potential plagiarism or unauthorized use of unpublished manuscripts, as outlined in the submission guidelines which state that this journal does not accept previously published manuscripts.