PENENTUAN STRUKTUR SENYAWA ANTIOKSIDAN LIMONOID DARI BIJI JERUK SAMBAL (Citrus microcarpa Bunge) KALIMANTAN BARAT

Authors

  • Setyo Widayanti Program Studi Pascasarjana Kimia, Fakultas MIPA, Universitas Tanjungpura
  • Rudiyansyah Rudiyansyah Program Studi Pascasarjana Kimia, Fakultas MIPA, Universitas Tanjungpura
  • Andi Hairil Alimuddin Program Studi Pascasarjana Kimia, Fakultas MIPA, Universitas Tanjungpura

DOI:

https://doi.org/10.26418/indonesian.v1i3.34193

Abstract

Limonoid has been isolated from orange seeds of Citrus microcarpa Bunge using extraction and partitioning methods. It is a yellowish-white crystal with a melting point of 276-277 oC. Based on the phytochemical analysis and FTIR spectroscopy, 1H NMR and compared with the literature, the compound is limonin which is a triterpenoid. The purpose of this study was to determine the structure and evaluate antioxidant activity of the limonin. The antioxidant activity by DPPH obtained IC50 value of limonin was 199.18 ppm. Whereas, the test antioxidant activity by FRAP method using a comparative solution of ascorbic acid showed that there was an increasingly blue color change, which meant that antioxidant activity was stronger with activity value of 11.88 mgAAE /g sample.

References

Sikander, Dar M. (2016). Study of Phytoconstituents, antioxidant and Antibacterial Activities of Citrus karna Fruits Peels. Thesis. Technology and Sciences, Allahabad: India.

Kemenristekdikti. (2017). Jeruk. http://migroplus.com/brosur/Budidaya%20 jeruk.pdf, diakses tanggal 02 Februari 2017.

Al-Snafi, A. Esmail. (2016). Nutritional value and pharmacological importance of citrus species grown in Iraq. Journal of Pharmacy, 6(8), 76-108.

Malee, Tareeyah. (2011). Chemical Constituents from the Leaves and Stems of Citrus aurantifolia Swingle. Thesis. Master of Science Degree. Prince of Songkla University: Songkla.

Kranthi K. Mandadia,b, Guddadarangavvanahally K. Jayaprakashaa,b, Narayan G. Bhatc, dan Bhimanagouda S. Patila. (2006). Red Mexican Grapefruit: A Novel Source for Bioactive Limonoids and their Antioxidant Activity. Vegetable and Fruit Improvement Center, 62c, 179-188.

Can Liu, Jing Liu, Yonghai Rong, Nvyong Liang dan Long Rong. (2012). Aqueous extraction of limonin from Citrus reticulate Blanco. Czech J. Food Sci, 4(30), 364–368.

Bauri, A. K., Sabine Foro dan Quynh Nguyen Do Nhu. (2016). Limonin isolated from the seeds of Citrus limetta (Indian sweet lemon). IUCrData, 1, x160172.

Anshori, J., Al, Euis Julaeha, Tri Mayanti, Unang Supratman, Hideo Hayashi. (2006). Senyawa Limonin dari Biji Jeruk Citrus nobilis var. Microcarpa (Jeruk Siam) dan Potensi Aktivitasnya sebagai Penghambat Tumbuh Larva Instar ke-empat Nyamuk Aedes Aegypti, Jurnal Riset dan Teknologi Pembangunan Perekonomian.

Fajarwati, N. (2013). Uji Aktivitas Antioksidan Pada Ekstrak Daun Jeruk Nipis (Citrus aurantifolia) Dengan Menggunakan Metode DPPH (1,1-DIPHENYL-2-PICRYLHYDRAZYL). Skripsi. FKIK. UIN Syarif Hidayatullah: Jakarta.

Wulandari, M., Nora Idiawati, Gusrizal. (2013). Aktivitas Antioksidan Ekstrak n-Heksana, Etil Asetat dan Metanol Kulit Buah Jeruk Sambal (Citrus microcarpa Bunge). JKK. 2(2), 94-94.

Bui, K. A., Duong, A. T., Tran, V. S., Seip, S. (2004). Isolation and structure elucidation of a new limonoid from Vietnamese Citrus nobilis seeds. Tap. Chi. Hoa. Hoc., 42(4), 520-523.

Chu, J., Song-Lin Li, Zhi-Qi Yin, Wen-Cai Ye, Qin-Wen Zhang. (2012). Simultaneous Quantification of Coumarins, Flavonoids, and Limonoids in Fructus Citri Sarcodactylis by High Performance Liquid Chromagraphy Coupled With Diode Array Detector. Journal of Pharmaceutical and Biomedical analysis. 66, 170-175.

Hasegawa, S., raymond D. Bennett, dan Carl P. Verdon. (1980). Metabolism of Limonoids Via a Deoxylimonoid Pathway in Citrus. Phytochemistry. (19), 1445-1447.

Huang D., Boxin Ou, dan Ronald L. Prior. (2005). The Chemistry behind Antioxidant Capacity Assays. Journal Agric. Food Chem., 6(53), 1841-1856.

.

Downloads

Published

2019-07-12

Issue

Vol. 1 No. 3 (2018)

Section

Articles

License

Copyright (c) 2019 Setyo Widayanti, Rudiyansyah Rudiyansyah, Andi Hairil Alimuddin

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Authors who publish with this journal agree to the following terms: 1. Authors retain copyright and grant the journal the right of first publication, with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal. 2. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal. 3. Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (see The Effect of Open Access).