KARAKTERISASI SENYAWA FENOLIK DARI BIJI BUAH RAMBUTAN (Nephelium lappaceum L.)

Authors

  • Eka Pebri Malinda Program Studi Kimia, Fakultas MIPA, Universitas Tanjungpura
  • Rudiyansyah Rudiyansyah Program Studi Kimia, Fakultas MIPA, Universitas Tanjungpura
  • Ajuk Sapar Program Studi Kimia, Fakultas MIPA, Universitas Tanjungpura

DOI:

https://doi.org/10.26418/indonesian.v2i3.36890

Abstract

Rambutan seeds are one of the waste produced from rambutan that is potential to be used in the food and pharmaceutical fields because it is rich in nutrients and secondary metabolites. This research was carried out to characterize phenolic compounds from the ethyl acetate fraction by using chromatographic methods, phytochemical tests, and 1H-NMR analysis. The isolate was obtained as white powder with 6.2 mg and gave positive sign for phenolic by phytochemical test. 1H-NMR spectrum data (CD3OD, 500 MHz) showed the chemical shift characteristic of coumarin compounds at δH (ppm): 7.82 (1H, d, J= 8.3 Hz), 7.39 (1H, d, J= 7.55 Hz), 6.73 (1H, d, J= 8.05 Hz), and 5.61 (1H, d, J= 7.55 Hz), an anomeric proton at δH 4.8-5 ppm, the characteristics of the methyl group of L-rhamnose sugar at δH 1.29 ppm and other sugar protons at δH 3-4 ppm. Based on the 1H-NMR analysis and comparison with literature, a phenolic compound obtained from rambutan (Nephelium lappaceum L.) seeds is                                                      7-O-β-L-rhamnose-8-hydroxycoumarin or 8-O-β-L-rhamnose-7-hydroxycoumarin.

References

Mahisanunt,B., Najom,K., Matsukawa,S., dan Klinkesorn,U. (2017). Solvent Fractionation of Rambutan (Nephelium lappaceum L.) Kernel Fat for Production of Non-Hydrogenated Solid Fat: Influence of Time and Solvent Type. Journal of King Saud University Science, 29, 32-46.

Thitilertdecha,N., Teerawutgulajaga, A., dan Rakariyatham,N. (2008) Antioxidant and Antibacterial Activities of Nephelium lappaceum L. Extracts. Food Science and Technology, 41, 2029-2035.

Aprilia,F,P. (2010). Uji Aktivitas Antidiabetes Ekstrak Air Biji Rambutan (Nephelium lappaceum L.) pada Mencit Diabetes Mellitus Akibat Induksi Aloksan. (Skripsi), Universitas Jember, Fakultas Farmasi, Jember.

Yuda,A.A.G.P., Rusli,R., dan Ibrahim,A. (2015). Kandungan Metabolit Sekunder dan Efek Penurunan Glukosa Darah Ekstrak Biji Rambutan (Nephelium lappaceum L.) pada Mencit (Mus musculus). Jurnal Sains dan Kesehatan, 1 (3), 120-125.

Sukmawati,S.H., Harlia, dan Rudiyansyah. (2017). Karakterisasi Struktur Senyawa Kumarin Glikosida dari Biji Buah Rambutan (Nephelium lappaceum L.). Jurnal Kimia Khatulistiwa, 6 (3),1-5.

Mulia, J., Rudiyansyah, dan Wibowo, M.A. (2019). Karakterisasi Senyawa Fenolik dari Biji Buah Rambutan (Nephelium lappaceum L.). Jurnal Kimia Khatulistiwa, 8 (1), 26-31.

McHale,D., Khopkar,P.P., dan Shendan,J.B. (1987) Coumarin Glycosides from Citrus flavedo. Phytochemistry, 26 (9), 2547-2549.

Guo, Q., Ai, L. dan Cui, S. (2018) Methodology for Structural Analysis of Polysaccharides. Springer. https://doi.org/10.1007/978-3-319-96370-9_7.

Yamada,K., Teshima,T., dan Yamamoto,T. (2015). Methode for Imaging Cell Using Fluorescence-Labeled Sugar Derivate Having Coumarin Derivate Bound Thereo, and Imaging Agent. US Patent Application Publication. US 2015/0369797 A1.

Alegantina, S. dan Isnawati, A. (2010). Identifikasi dan Penetapan Kadar Senyawa Kumarin dalam Ekstrak Metanol Artemisia annua L. secara Kromatografi Lapis Tipis-Densitometri. Bul. Penelit. Kesehatan, 38 (1), 17-28.

Downloads

Published

2019-10-10

Issue

Vol. 2 No. 3 (2019)

Section

Articles

License

Copyright (c) 2019 Eka Pebri Malinda, Rudiyansyah Rudiyansyah, Ajuk Sapar

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Authors who publish with this journal agree to the following terms: 1. Authors retain copyright and grant the journal the right of first publication, with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal. 2. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal. 3. Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (see The Effect of Open Access).