DAYA HAMBAT EKSTRAK DAUN MANGROVE (Rhizophora apiculata) ASAL PESISIR SUKADANA TERHADAP BAKTERI Escherichia coli (INHIBITION THE LEAF EXTRACT OF Rhizophora apiculata FROM THE SUKADANA COAST AGAINST Escherichia coli)

Authors

  • Muhamad Agus Wibowo Jurusan Kimia, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Tanjungpura, Indonesia
  • Ninda Kumala Sari Jurusan Kimia, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Tanjungpura, Indonesia
  • Puji Ardiningsih Jurusan Kimia, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Tanjungpura, Indonesia
  • Afghani Jayuska Jurusan Kimia, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Tanjungpura, Indonesia

DOI:

https://doi.org/10.26418/indonesian.v6i3.69965

Abstract

Rhizophora apiculata is a plant that is widely found in mangrove forests in Sukadana subdistrict, and has the potential to inhibit of Escherichia coli. This research aims to determine the activity of R. apiculata which grows in the Sukadana mangrove forest in inhibiting E. coli. The leaves of R. apiculata were macerated with methanol solvent, partitioned into three fractions (n-hexane fraction, dichloromethane fraction, and methanol fraction), and tested for phytochemical screening. The results of phytochemical screening of R. apiculata leaves were positive for flavonoids, alkaloids, phenolics, terpenoids, tannins, and saponins. The potential of R. apiculata leaf samples in inhibiting bacterial growth was measured by the size of the clear zone in the antibacterial test with the agar well diffusion method that uses variations in concentration, namely 250 mg/mL, 125 mg/mL, and 62.5 mg/mL. Based on the research results, it is known that R. apiculata leaves can inhibit the growth of the test bacteria with a minimum inhibitory concentration for methanol extract of 250 mg/mL. The methanol and dichloromethane fractions had minimum inhibitory concentrations of 125 mg/mL and 250 mg/mL respectively, while the n-hexane fraction did not inhibit at all test concentrations. So it can be concluded that the leaves of R. apiculata have the potential as an alternative for antibacterial active compounds

References

Harnanda, F., Rafdinal, Linda, R. Komposisi dan Tingkat Kerusakan Vegetasi Hutan Mangrove di Kecamatan Sukadana Kabupaten Kayong Utara Provinsi Kalimantan Barat. Jurnal Protobiont [Internet]. 12 Januari 2018 [dikutip 28 Juli 2023];7(1). Tersedia pada: https://jurnal.untan.ac.id/index.php/jprb/article/view/23628

Apriani A, Akbar AA, Jumiati J. Valuasi Ekosistem Mangrove di Pesisir Kayong Utara, Kalimantan Barat. Jurnal Ilmu Lingkungan. 6 Juli 2022;20(3):553–62.

Wardina MA, Mustofa S, Malarangeng ANTA. Review Article: Potensi Rhizophora apiculata Sebagai Fitofarmaka. Medical Profession Journal of Lampung. 17 Februari 2023;13(2):137–46.

Rajendra S, Karthick P, Kada NM, CH R, Mohanraju R, Annamalai V. Evaluation of antimicrobial properties from the mangrove Rhizophora apiculata and Bruguiera gymnorrhiza of Burmanallah coast, South Andaman, India. Journal of Coastal Life Medicine. 1 Juni 2016;4:475–8.

Usman U. Phytochemical Test and Antibacterial Test of Rhizopora apiculata Mangrove-Root against Escherichia coli and Staphylococcus aureus Bacteria. JKPK (Jurnal Kimia dan Pendidikan Kimia). 3 Januari 2018;2(3):169–77.

Ergina E, Nuryanti S, Pursitasari ID. Uji Kualitatif Senyawa Metabolit Sekunder Pada Daun Palado (Agave Angustifolia) Yang Diekstraksi Dengan Pelarut Air Dan Etanol. Jurnal Akademika Kimia. 21 Februari 2017;3(3):165–72.

Fajriaty I, I.h H, Saputra IR, Silitonga M. Skrining Fitokimia dan Analisis Kromatografi Lapis Tipis dari Ekstrak Etanol Buah Lerak (Sapindus rarak). Jurnal Pendidikan Informatika dan Sains. 27 Desember 2017;6(2):243–56.

Manongko PS, Sangi MS, Momuat LI. Uji Senyawa Fitokimia dan Aktivitas Antioksidan Tanaman Patah Tulang (Euphorbia tirucalli L.). Jurnal MIPA. 29 Mei 2020;9(2):64–9.

Fardin F, Wulan C. UJI AKTIVITAS ANTIBAKTERI EKSTRAK METANOL JAMUR RAYAP (Termitomyces albuminosus (Berk.) Heim.) TERHADAP BAKTERI Staphylococcus aureus dan Bacillus subtilis. Majalah Farmasi Nasional. 30 Agustus 2016;13(2):46–54.

Akasia AI, Putra IDNN, Putra ING. Skrining Fitokimia Ekstrak Daun Mangrove Rhizophora mucronata dan Rhizophora apiculata yang Dikoleksi dari Kawasan Mangrove Desa Tuban, Bali. Journal of Marine Research and Technology. 28 Februari 2021;4(1):16–22.

Mutik MS, Sibero MT, Widianingsih W, Subagiyo S, Pribadi R, Haryanti D, dkk. Kandungan Senyawa Bioaktif dan Aktivitas Biologis Ekstrak Daun Rhizophora apiculata Asal Perairan Teluk Awur, Jepara. Jurnal Kelautan Tropis. 1 November 2022;25(3):378–90.

Å tumpf S, Hostnik G, PrimožiÄ M, Leitgeb M, Bren U. Generation Times of E. coli Prolong with Increasing Tannin Concentration while the Lag Phase Extends Exponentially. Plants (Basel). 1 Desember 2020;9(12):1680.

Å tumpf S, Hostnik G, PrimožiÄ M, Leitgeb M, Salminen JP, Bren U. The Effect of Growth Medium Strength on Minimum Inhibitory Concentrations of Tannins and Tannin Extracts against E. coli. Molecules. 26 Juni 2020;25(12):2947.

Hamdi Abdulkareem M, Abbas Abood I, Munis Dakheel M. Antimicrobial Resistance of Tannin Extract against E. coli Isolates from Sheep. Arch Razi Inst. 30 April 2022;77(2):697–701.

Kaczmarek B. Tannic Acid with Antiviral and Antibacterial Activity as A Promising Component of Biomaterials—A Minireview. Materials (Basel). 20 Juli 2020;13(14):3224.

Olchowik-Grabarek E, Sekowski S, Bitiucki M, Dobrzynska I, Shlyonsky V, Ionov M, dkk. Inhibition of interaction between Staphylococcus aureus α-hemolysin and erythrocytes membrane by hydrolysable tannins: structure-related activity study. Scientific Reports. 7 Juli 2020;10.

Shinde DS, Vs S, Khairnar S. A Short Review on the Improvement of Antimicrobial Activity by Metal and Nonmetal Doping in Nanoscale Antimicrobial Materials. 21 Juli 2019;163.

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Published

2023-10-30

Issue

Vol. 6 No. 3 (2023)

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Articles

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Copyright (c) 2023 Muhamad Agus Wibowo, Ninda Kumala Sari, Puji Ardiningsih, Afghani Jayuska

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