Isolasi, Identifikasi dan Aktivitas Antibakteri Bakteri Endofit Daun Pegagan (Centella asiatica L.) terhadap Escherichia coli
DOI:
https://doi.org/10.26418/jc.v5i4B.44821Keywords:
Centella asiatica L., Bakteri endofit, Escherichia coli, zona hambatAbstract
Latar Belakang. Di Indonesia diare masih merupakan masalah kesehatan masyarakat. Tanaman pegagan telah digunakan untuk pengobatan diare. Bakteri endofit adalah bakteri yang hidup di dalam jaringan tanaman inang. Beberapa jenis bakteri endofit mampu menghasilkan senyawa aktif yang bersifat antibiotik. Metode. Penelitian ini merupakan penelitian deskriptif-eksploratif. Isolasi bakteri endofit dari daun pegagan (C.asiatica L.) menggunakan metode tanam langsung, potensi antibakteridiuji dengan metode difusi cakram. Identifikasi bakteri endofit dilakukan dengan pengamatan morfologi koloni, morfologi sel dan uji biokimia. Hasil. Sebanyak 4 dari 42 isolat memiliki potensi sebagai antibakteri terhadap pertumbuhan bakteri Escherichia coli. Isolat 16 memiliki kemampuan tertinggi yaitu dengan zona hambat 6,5 mm. isolat 16 memiliki kemiripan dangan genus Pseudomonas. Kesimpulan. Sebanyak 4 isolat bakteri endofit yang mempunyai potensi antibakteri terhadap Escherichia coli
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