Efek protektif kombinasi minyak jintan hitam (Nigella sativa) dan madu pada ginjal tikus yang diberi pajanan cisplatin
DOI:
https://doi.org/10.26418/jc.v6i4.47803Keywords:
antioksidan, minyak jintan hitam, madu, cisplatin, sinergismeAbstract
Latar belakang: Cisplatin merupakan agen antikanker dengan berbagai efek samping, terutama nefrotoksisitas. Minyak jintan hitam (MJH) dan madu (M) merupakan bahan alam kaya akan antioksidan dan memiliki efek nefroprotektif. Penelitian ini bertujuan untuk mengetahui efek nefroprotektif kombinasi minyak jintan hitam dan madu pada tikus yang dipajan cisplatin. Metode: Penelitian ini menggunakan 30 tikus yang dibagi menjadi 10 kelompok, yaitu kelompok kontrol (K), kelompok tunggal MJH dosis I (1 mL/kgBB) (P1), MJH dosis II (2 mL/kgBB) (P2), M dosis I (3,7 mL/kgBB) (P3), M dosis II (7,4 mL/kgBB) (P4), kelompok kombinasi MJH dosis I dan M dosis I (P5), MJH dosis I dan M dosis II (P6), MJH dosis II dan M dosis I (P7), MJH dosis II dan M dosis II (P8) serta kelompok cisplatin (C). Minyak jintan hitam dan madu diberikan selama 21 hari. Pada hari ke-18, semua kelompok, kecuali kelompok kontrol, diberi cisplatin 8 mg/kgBB secara intraperitoneal. Hasil: Terdapat perbedaan bermakna pada skor tubulus proksimal normal semua kelompok perlakuan (kecuali P3) dibandingkan dengan kelompok cisplatin (p<0,05). Kombinasi minyak jintan hitam dan madu menghasilkan efek yang sinergis (CI = 0,234, 0,537, 0,863, 0,586). Kesimpulan: Kombinasi minyak jintan hitam dan madu menghasilkan efek protektif yang sinergis terhadap jaringan ginjal tikus yang diberi pajanan cisplatin.
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