Optimasi pengaruh ekstrak etanol 70 % bebas dan tidak bebas alkaloid daun Justicia gendarussa Burm. F terhadap enzim reverse transcriptase HIV in vitro

Authors

  • Bambang Prayogo Universitas Airlangga
  • Prihartini Widiyanti Universitas Airlangga
  • Hafrizal Riza

DOI:

https://doi.org/10.26418/jurkeswa.v1i2.42998

Keywords:

Justicia gandarussa, ekstrak 70% etanol bebas dan tidak bebas alkaloid, reverse transcriptase, anti-HIV

Abstract

Latar Belakang:  Ekstrak etanol 70% daun Justicia gendarussa Burm.f, bebas dan tidak bebas alkaloid  dipercaya memiliki aktivitas inhibisi yang kuat terhadap enzim Reverse Transcriptase HIV, sebagai  upaya menemukan solusi dalam menghadapi prevalensi HIV AIDS yang masih tinggi dengan kendala  pengobatan HIV AIDS seperti efeks samping dan resistensi. Metode: Kedua jenis ekstrak diujikan  secara in vitro menggunakan teknik Elisa dan dianalisa kandungan kimia yang Gendarusin A yang  memiliki efek antiHIV menggunakan kromatografi cair kinerja tinggi. Hasil: Hasil uji Elisa diperoleh  persen penghambatan berturut-turut sebesar , 254,2, 254,2, 235,6 dan 279,7 untuk konsentrasi 5 ppm,  10 ppm, 15 ppm dan 20 ppm ekstrak bebas alkaloid dan sebesar169,0, 164,0, 130,5 dan 369,5 untuk  konsentrasi 5 ppm, 10 ppm, 15 ppm dan 20 ppm ekstrak tidak bebas alkaloid. Hasil kandungan kimia  diperoleh Gendarusin A pada ekstrak bebas bebas alkaloid pada waktu retensi 8,402 menit dan ekstrak  tidak bebas alkaloid pada waktu retensi 8,381. Kesimpulan: Justicia gendarussa Burm.f dapat  menjadi sumber yang bermanfaat untuk isolasi dan pengembangan inhibitor baru antiHIV.

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Published

2015-04-01

Issue

Vol. 1 No. 2 (2015)

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Articles

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