TA CLONING PARTIAL ALBUMIN GENE AND ITS TEST FOR SUPPORTING PHARMACOGENOMIC DETECTION DEVELOPMENT

Authors

  • Rinal Effendi Andalas University
  • Ajeng Putri Kumala Genetic Engineering Research Center, Biological and Environmental Organization, BRIN
  • Inherni Marti Abna Esa Unggul University, Jakarta, Indonesia
  • Oktri Yurika Genetic Engineering Research Center, Biological and Environmental Organization, BRIN
  • Desriani Desriani Genetic Engineering Research Center, Biological and Environmental Organization, BRIN

DOI:

https://doi.org/10.26418/jpmipa.v15i3.85093

Keywords:

Albumin gene, PCR (Polymerase Chain Reaction), Pharmacogenomic detection, TA cloning, Vektor pGEM- T Easy

Abstract

DNA recombinant technology is a tool that could support thedevelopment of pharmacogenomic detection such as for CYP2D6 copy number detection application. The housekeeping gene was identified as an internal control alternative for the copy number of the CYP2D6 detection component. There are many kinds of housekeeping genes, including albumin. In this report, we have successfully TA-cloned partial albumin to support the development of CYP2D6 copy number detection. TA-cloning was successfully confirmed with white-blue methods in media LB + ampicillin + IPTG + X-Gal and with colony PCR methods. Further, we made a standard curve with this recombinant plasmid to evaluate albumin qPCR efficiency and showed high-efficiency qPCR 0,9914 (99%). Based on the CYP2D6 copy number developed, the test in this study showed low concordance with high reproducibility Long PCR, indicating that the nucleotide base area of albuminand CYP2D6 in this studywas not recommended for CYP2D6 gene multiplication detection.  

Author Biographies

Rinal Effendi, Andalas University

Department of Anesthesia, Faculty of Medicine, Andalas University, Limau Manis Campus, Padang, West Sumatra, Indonesia

Ajeng Putri Kumala, Genetic Engineering Research Center, Biological and Environmental Organization, BRIN

BRIN. KST Ir Soekarno Hatta Jl Raya Cibinong Km 46, Cibinong-West Java, Indonesia

Inherni Marti Abna, Esa Unggul University, Jakarta, Indonesia

Department of Pharmacy, Faculty of Health Sciences, Esa Unggul University, Jakarta, Indonesia

Oktri Yurika, Genetic Engineering Research Center, Biological and Environmental Organization, BRIN

BRIN. KST Ir Soekarno Hatta Jl Raya Cibinong Km 46, Cibinong-West Java, Indonesia

Desriani Desriani, Genetic Engineering Research Center, Biological and Environmental Organization, BRIN

BRIN. KST Ir Soekarno Hatta Jl Raya Cibinong Km 46, Cibinong-West Java, Indonesia

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Published

2024-09-12

Issue

Vol. 15 No. 3 (2024): September 2024

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