SCREENING AND DETERMINATION OF EMULSIFICATION ACTIVITY OF SURFACTANT-PRODUCING BACTERIA FROM PALM OIL INDUSTRY LIQUID WASTE

Authors

  • Vida Elsyana Politeknik Negeri Lampung https://orcid.org/0000-0001-8366-7203
  • Livia Rhea Alvita Politeknik Negeri Lampung
  • Dian Ayu Afifah Politeknik Negeri Lampung
  • Niken Feladita Politeknik Negeri Lampung
  • Panji Setiawan Politeknik Negeri Lampung

DOI:

https://doi.org/10.26418/jpmipa.v16i2.90726

Keywords:

Biosurfactant, oil spreading assay, emulsification acivity

Abstract

Biosurfactants are natural surfactants produced by certain microorganisms (bacteria, fungi, and yeast) and are considered the best candidates for replacing synthetic surfactants. Bacteria isolated from oil spill areas have shown the ability to produce biosurfactants. This study enriched bacteria from anaerobic wastewater of palm oil industry in Lampung then isolated and screened natural surfactant-producing bacteria and measured their emulsification activity. Screening of surfactant-producing bacteria was carried out using oil spreading assay and their emulsification activity was measured based on the emulsification index (EI). The results of the study obtained 7 bacterial isolates and all showed positive results in the oil spreading assay. The highest emulsification index (EI) was produced by the fifth isolate (LAC5) with a value of 42.32%. These results show the potential for emulsification activity of biosurfactants produced by anaerobic wastewater bacteria from palm oil industry ponds.

Author Biographies

Vida Elsyana, Politeknik Negeri Lampung

Study Program of Applied Chemistry, Department of Agricultural Technology

Livia Rhea Alvita, Politeknik Negeri Lampung

Study Program of Applied Chemistry, Department of Agricultural Technology

Dian Ayu Afifah, Politeknik Negeri Lampung

Study Program of Industrial Chemical Engineering Technology, Department of Engineering

Niken Feladita, Politeknik Negeri Lampung

Study Program of Applied Chemistry, Department of Agricultural Technology

Panji Setiawan, Politeknik Negeri Lampung

Study Program of Industrial Chemical Engineering Technology, Department of Engineering

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Published

2025-05-14

Issue

Vol. 16 No. 2 (2025): May 2025

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Articles

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