UJI EFEK EKSTRAK N-HEKSANA DAUN BAWANG KUCAI (Allium tuberosum Rottl ex Spreng) TERHADAP KOLESTEROL TOTAL PADA TIKUS PUTIH (Rattus norvegicus) JANTAN GALUR WISTAR

Abstract

Background: Garlic chives/kucai (Allium tuberosum Rotll ex Spreng)
contain Allicin compound. The Allicin compound has an inhibitory effect for
HMG-CoA reductase enzyme so that the cholesterol level was decreased.
Objective: The research would investigate the effect of reduction total
cholesterol and effective dose of garlic chives"™s n-hexane extract in male
wistar rate compared with atorvastatin as possitive control. Method: The
research was a time series experiment. Twenty five wistar rat was
randomly divided into 5 experimental group, negative control (CMC 1%),
positive control (atorvastatin), dose I   (0,0225 g/200 gBW), dose II (0,045
g/200 gBW), and dose III (0,09 g/200 gBW) that was induced by high
cholestrol diet for 14 days. The resulting data is analyzed with One-way
Anova test continued with LSD Post Hoc testing. Result: From
phytochemical testing result, garlic chives n-hexane extract contain
steroid/terpenoid secondary metabolite. Statistical analysis showed
significance difference between average cholestrol level from control
group (negative and positif) againts dose I group and dose II group
(p<0,05) at day-42. The effective dose was 0,09 g/200 gBB Conclusion:
N-hexane extract of garlic chives has total cholestrol reduction effect on
white male wistar rat with effective dose at 0,09 g/200 gBW which show
the same effectivity with atorvastatin.

Key Words: Allium tuberosum Rottl ex Spreng, n-hexane extract of garlic
chives, high cholestrol diet, total cholestrol level lowering effect

1) Medical School, Faculty of Medicine, Universitas Tanjungpura
Pontianak, Kalimantan Barat
2) Pharmacy School, Faculty of Medicine, Universitas Tanjungpura
Pontianak, Kalimantan Barat
3) Departement of Biochemistry, Medical School, Faculty of Medicine,
Universitas Tanjungpura Pontianak, Kalimantan Barat