UJI AKTIVITAS ANTIBAKTERI FRAKSI n-HEKSANA KULIT BUAH MANGGIS (Garcinia mangostana L.) TERHADAP PERTUMBUHAN Shigella flexneri SECARA IN VITRO

Abstract

Background: diarrhea is one of the health problems that still needs more
attention especially in developing countries such as Indonesia. Shigella
flexneri is a bacterial isolates were found in most cases of diarrhea. Based
on scientific information about Garcinia mangostana L., this plant has a
potentcy   as an alternative treatment of antibacterial. Objective: the aimed
of this study was to determined the antibacterial activity of n-hexane fraction
of mangosteen peels (Garcinia mangostana L.) and the effective
concentration to inhibit growth of Shigella flexneri Method: mangosteen
peels were extracted by maceration method using 90% methanol then this
extracts was fractionated using n-hexane. The chemical compound of nheksane

fraction was screened by phytochemical screening. Antibacterial
activity was conducted by using well diffusion with concentration of 5%,
10%, 20%, and 40%. Ciprofloxacin 5μg/hole was used as positive control
and DMSO 10% was used as negative control. Result: the phytochemical
screening of n-heksana fraction of mangosteen peels is contains alkaloids,
flavonoids, quinones and terpenoids. Concentration of 40% is the highest
concentration that has great antibacterial effect of all various concentration
tests but this concentration only moderate inhibition zone (p=0,000). The
inhibition zone of each concentration has decreased at 48 hours incubation.
Conclusion: n-hexane fraction of mangosteen peels has antibacterial
activity against Shigella flexneri.
.  
Keywords: Antibacterial, n-heksane fractions of mangosteen peels,
Shigella flexneri

1) Medical Education Study Program, Faculty of Medicine, Tanjungpura
University, Pontianak, West Kalimantan
2) Biology Study Program, Faculty of Mathematics and Natural Science,
Tanjungpura University, Pontianak, West Kalimantan
3) Department of Physiology, Medical Education Study Program, Faculty
of Medicine, Tanjungpura University, Pontianak, West Kalimantan