STUDI AWAL PEMISAHAN AMILOSA DAN AMILOPEKTIN PATI UBI JALAR (Ipomoea batatas Lam) DENGAN VARIASI KONSENTRASI n-BUTANOL

Authors

  • Astrid Devita Oktavia, Nora Idiawati, Lia Destiarti

Abstract

Penelitian tentang studi awal pemisahan amilosa dan amilopektin pati ubi jalar (Ipomoea batatas Lam) telah dilakukan dengan variasi konsentrasi n-butanol. Penelitian ini bertujuan untuk memperoleh hasil analisis proksimat. Rendemen fraksi amilosa dan amilopektin pati ubi jalar yang difraksinasi dengan variasi konsentrasi 10%, 20%, 30% dan 40% n-butanol, serta mengkarakterisasi senyawa amilopektin dari pati ubi jalar dengan spektrofotometer IR. Hasil analisis proksimat pati ubi jalar menunjukkan kandungan air 61,51%, abu 1,7%, lemak 2,12%, protein 1,1%, dan karbohidrat 33,57%. Identifikasi amilosa dengan iodin menghasilkan warna biru keunguan, sedangkan amilopektin menghasilkan warna coklat kemerahan. Persentase rendemen fraksi amilosa tertinggi berada pada konsentrasi n-butanol 40% sebesar 46,69% dan fraksi amilopektin optimum berada pada konsentrasi 30% n-butanol sebesar 52,25%. Spektrum fraksi amilopektin menunjukkan serapan pada bilangan gelombang 3425,34 (uluran O−H), 1647,10 (uluran C=O), 1022,20-1157,21 (uluran C−O), 522,67-609,46 (uluran C−C−O), 435,88 (uluran C−C−C). Adanya gugus karbonil (C=O) pada spektrum tersebut disebabkan proses oksidasi dari amilopektin.

Kata kunci : Ipomoea batatas Lam, amilosa, amilopektin, fraksinasi

Downloads

Published

2013-11-30

Issue

Vol. 2 No. 3 (2013): Jurnal Kimia Khatulistiwa

Section

Articles

License

Authors who publish with this journal agree to the following terms:

  1. Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a  Creative Commons Attribution License  that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
  2. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
  3. Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See  The Effect of Open Access).