KARAKTERISASI METABOLIT SEKUNDER FRAKSI N-HEKSAN SPONS Aaptos suberitoides ASAL PERAIRAN PULAU LEMUKUTAN KALIMANTAN BARAT
Abstract
Spons Aaptos suberitoides merupakan biota laut dari kelas Demospongiae dan menjadi sumber bahan baku obat. Beberapa penelitian menyatakan bahwa spons Aaptos mengandung senyawa metabolit sekunder seperti alkaloid, steroid dan terpenoid. Penelitian ini bertujuan untuk mengetahui jenis metabolit sekunder dari fraksi n-heksan spons Aaptos suberitoides menggunakan FTIR, NMR dan GCMS. Metode yang digunakan adalah uji fitokimia, KLT, KKF dan KLT 2 Dimensi serta identifikasi dengan instrumen. Berdasarkan hasil uji fitokimia menunjukkan kandungan metabolit sekunder dari alkaloid dan steroid. Hasil karakterisasi senyawa dengan FTIR menunjukkan gugus fungsi OH, aromatik, CH alifatik, C=C, cincin aromatik C=C-C, CH, ester aromatik, dan CO3. Analisis 1HNMR (CDCl3, 500 MHz) diperoleh geseran kimia δ 0.82 ppm (1H, m), δ 0.90 ppm (5H, m), δ 0.95 (1H, s), δ 1.15 ppm (1H, s), δ 1.27 ppm (4H, s), δ 1.31 ppm (12H, qd), δ 1.32 ppm (2H, qd), δ 1.37 ppm (1H, d), δ 1.40 ppm (1H, m), δ 1.45 ppm (1H, m), δ 1.69 ppm (1H, p), δ 4,25 ppm (3H, m), δ 7.54 ppm (1H, dd) dan δ 7.72 (1H, dd). Berdasarkan geseran kimia isolat cenderung memiliki kemiripan terhadap senyawa 2-etil-1-hidroksi heksil (2-etil heksil) ftalat. Berdasarkan hasil analisis GCMS dari isolat senyawa utamanya adalah bis (2-etil heksil) ftalat (46.37%).References
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- KARAKTERISASI METABOLIT SEKUNDER FRAKSI N-HEKSAN SPONS Aaptos suberitoides ASAL PERAIRAN PULAU LEMUKUTAN KALIMANTAN BARAT
- KARAKTERISASI METABOLIT SEKUNDER FRAKSI N-HEKSAN SPONS Aaptos suberitoides ASAL PERAIRAN PULAU LEMUKUTAN KALIMANTAN BARAT
- KARAKTERISASI METABOLIT SEKUNDER FRAKSI N-HEKSAN SPONS Aaptos suberitoides ASAL PERAIRAN PULAU LEMUKUTAN KALIMANTAN BARAT
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