OPTIMASI SUHU ANNEALING GEN mecA RESISTENSI ANTIBIOTIK AMOKSISILIN DARI BAKTERI Staphylococcus aureus PADA PASIEN ULKUS DIABETIK

Abstract

Amoxicillin antibiotic resistance can caused by Staphylococcus aureus bacteria that has a specific mecA gene inside chromosome and plasmid of bacteria. This gene can encode MRSA (Methicillin Resistant Staphylococcus aureus) which a form of antibiotic resistance, especially betalactam group. Quick and accurate detection is needed to determine the right antibiotic therapy for diabetic ulcer patients. The in vitro method that used is bacterial extraction with conventional kits and PCR (Polymerase Chain Reaction), but the success of PCR amplification is influenced by optimal annealing temperature in primary attachment process. Therefore, this study aims to optimize the annealing temperature to detect the mecA gene using a specific primer mecA gene forward 5'-AAAATCGATGGTAAAGGTTGGC-3 'and reverse 5'-AGTTCTGCAGTACCGGATTTGC-3' using annealing temperature 55 ° C for 30 seconds and 60 ° 60 C for 10 seconds. The results obtained that mecA gene in Staphylococcus aureus bacterial isolates of diabetic ulcer patients with a size of 533 bp using annealing temperature of 55 ° C for 30 seconds and no results were obtained at annealing temperature of 60 ° C for 10 seconds. Keyword: mecA gene, Polymerase Chain Reaction, Resistance, Staphylococcus aureus