UJI AKTIVITAS ANTIOKSIDAN EKSTRAK ETANOL DAUN BUASBUAS (Premna cordifolia Linn.) TERHADAP GAMBARAN HISTOPATOLOGI PARU TIKUS (Rattus norvegicus) WISTAR JANTAN PASCA PAPARAN ASAP ROKOK

Abstract

Cigarette smoke is one of free radical source that can cause oxidative
stress, emphysema, and inflamation on lung. Leaves of Premna cordifolia Linn. is
one of antioxidant that can neutralize the free radical in body. The purpose of the
research   is to find the antioxidant activity of Premna cordifolia leaves in reducing
lung"™s damage and to find the effective dose that can reduce lung"™s damage after
exposure of cigarette smoke. Dried leaves of Premna cordifolia are maserated
with ethanol 70%.   Rats are divided into five groups, Group without treatment
(CMC control), group that exposed to smoke, three groups that treated with 200
(P1), 400 (P2), and 600mg/kgBW (P3) of ethanolic extract of Premna cordifolia
leaves after smoke exposure, and one group that treated with 18mg/kgBW of
vitamin E as positive control after smoke exposure. All groups were treated with
three cigarette without filter for 14 days. All rat were terminated on the fifteent
day. Right lung were collected and subjected to necropsy and stained with
Hematoksilin-Eosin. The result show that all doses of ethanolic extract of Premna
cordifolia reduce lung damage in enlarge alveolar, thickening of alveolar"™s wall,
and infiltration of inflmation cells in rats. The effective dose is 600mg/kgbw
because the potential of Premna cordifolia leaves extract was same with the
potential of positive contol in reducing the lung damage.
Keywords : Antioxidant, Premna cordifolia, Lung histopathology, Cigarette
smoke