FORMULASI DAN UJI SIFAT FISIKOKIMIA SEDIAAN LOSIO DENGAN BERBAGAI VARIASI KONSENTRASI VITAMIN E

Abstract

Vitamin E is an antioxidant that can bind with oxygen to destroys free radicals. Vitamin E
in tissues suppress oxidation of unsaturated fatty acids, which helps and maintain cell membrane
function. This research was aimed to find out the optimum formulation of lotion vitamin E and
also to determine the levels of vitamin E in lotion once it was made. The formula of lotion was
made with series of five concentrations of vitamin E those are 1%, 2%, 3%, 4%, and 5%. The
optimum formula is chosen based on two physicochemical properties parameters, pH and
viscosity. Analysis of data was performed using program R i386 3.1.1 package R-commander. The
results showed that formula E (contained concentration 5%) was selected as the optimum formula
with spreading capacity of 6,97 cm; adhesive force 38,33 s; pH 7,38; and the viscosity 38,67 P.   C
column was used in this research for HPLC condition. Validation result from the analysis method
used standard levels series displaying correlation coefficient (r) of 0,9973; relative standard
deviation (RSD) ≤ 16 %; percentage of recovery 92,16 %; limit of detection (LOD) 1,725 µg/mL;
and limit of quantitation (LOQ) 5,752 μg/mL. The result of vitamin E levels obtained by HPLC
are 3,956 %.  
Keywords: Vitamin E, alpha tocopherol, Losio, Lotion, High Performance Liquid
Chromatography, HPLC