Aktivitas Biologis Asap Cair Batang Manggis (Garcinia mangostana L.) Terhadap Viabilitas Streptococcussp. (L.10.3)

Authors

  • Lilis Susanti Elvi Rusmiyanto Rikhsan Kurniatuhadi Jurusan Biologi - Fakultas MIPA Universitas Tanjungpura Jl. Prof. Dr. H. Hadari Nawawi Pontianak - Kalimantan Barat

DOI:

https://doi.org/10.26418/protobiont.v7i3.29062

Keywords:

Garcinia mangostana, Inhibition, Wood vinegar, Streptococcus sp., Viability

Abstract

The wood vinegar of the stem of mangosteen (G. mangostana L.) is the result of pyrolysis of the stem which is condensed into steam. Wood vinegar contains acid and phenol compounds that have antibacterial activities. This research aims to determine the biological activities of the wood vinegar of the stem of mangosteen (G. mangostana L.) on the viability of Streptococcus sp. (L.10.3) and determine the concentration of wood vinegar of the stem of mangosteen (G. mangostana L.) which is effective in inhibiting the growth of Streptococcus sp. (L.10.3). This research used a completely randomized design (CRD) with 6 treatments consisting of wood vinegar with a concentration of 0.1 (Tl); 0.5 (T2); 1 (T3) and 1.5% (T4), negative controls namely sterile distilled water (T5), and positive control namely chlorhexidine of 0.2% (T6). The viability test used the dilution method and calculation of the number of colonies using the total plate count (TPC) method, each treatment was repeated four times. The results showed the increasing concentration of wood vinegar of the stem of mangosteen (G. mangostana L.) and the decreasing amount of Streptococcus sp. (L.IO.3). Wood vinegar at concentration 1.5% showed bacterial growth of 6.9 x 105 CFU / ml which was very different from sterile distilled water by 2.5 x 107 CFU / ml with inhibition up to 96.9% and not significantly different from chlorhexidine of 0.2%.

Downloads

Published

2018-10-11

Issue

Vol. 7 No. 3 (2018)

Section

Articles

License

Authors who publish with this journal agree to the following terms:

  1. Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a  Creative Commons Attribution-ShareAlike License  that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
  2. Authors are not restricted by the journal from entering into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., posting it to an institutional repository or publishing it in a book), with an acknowledgement of its initial publication in this journal.
  3. Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) after its publication in this journal. This approach aims to safeguard against potential plagiarism or unauthorized use of unpublished manuscripts, as outlined in the submission guidelines which state that this journal does not accept previously published manuscripts.