STRUKTUR ANATOMI DAUN JERUK SIAM (Citrus nobilis L.var microcarpa) YANG BERGEJALA SAKIT

Abstract

Siamese orange (Citrus nobilis L. var microcarpa) is one of the fruit commodities in West Kalimantan. The growth of citrus plants in plantations can experience obstacles due to disease attacks caused by fungi and bacteria. Siamese orange leaves with symptoms of spot disease, cancer and scab can be distinguished based on leaf morphology. This study aims to determine the differences in the anatomical structure of leaves with symptoms of spots, cancer, and scab. This research was carried out for 3 months from March 2021 to May 2021. The paraffin method was used to make cross-sectional preparations of healthy citrus leaves and sick lime leaves using the paraffin method including fixation, washing, staining 1, dehydration, dealcoholization, paraffination, immersion, cutting, coloring 2, closing and labeling. The results showed that the anatomical structure of healthy siamese lime leaves showed that there were three tissue systems (dermal, ground tissue and vascular tissue) with clear shapes and boundaries, while in sick leaves, three tissue systems showed unclear boundaries in each tissue system. The anatomical structure of leaves with symptoms of spot disease, cancer and scab showed changes in cell shape and tissue layer thickness.