OPTIMASI KEPADATAN BAKTERI Serratia sp. VTS 1 (1) DAN LAMA INKUBASI TERHADAP KADAR AIR SAMPAH ORGANIK MENGGUNAKAN RESPONSE SURFACE METHODOLOGY (RSM)

Authors

  • Nurul Huda Tanjungpura University
  • Rahmawati Rahmawati Tanjungpura University
  • Novik Nurhidayat National Research and Innovation Agency

Keywords:

Serratia sp., RSM, Moisture Content, Organic Waste

Abstract

Organic waste contains a high water content, which can clog soil pores, hinder air circulation, and reduce oxygen availability. This leads to anaerobic decomposition, releasing compounds such as ammonia (NH3), which contribute to the pungent odours in organic waste. In this study, Serratia sp.VTS 1 (1), a bacterium isolated from vegetable waste, was used due to its potential for biodegrading organic waste. The aim of the research was to determine the optimal bacterial density and incubation time for reducing the water content of organic waste. The experimental design followed a Response Surface Methodology-Central Composite Design (RSM-CCD) with a factorial arrangement of 2 factors, each with 3 levels. Based on the contour plot of the moisture content response, the combination of a bacterial density of 109 CFU/mL and an incubation time of 7 days resulted in the lowest moisture content of organic waste, reaching 21%.

Author Biographies

Nurul Huda, Tanjungpura University

Department of Biology, Faculty of Mathematics and Natural Science, Tanjungpura University, Pontianak

Rahmawati Rahmawati, Tanjungpura University

Department of Biology, Faculty of Mathematics and Natural Science, Tanjungpura University, Pontianak

Novik Nurhidayat, National Research and Innovation Agency

Microbiological Recovery Research Group, Research Centre for Applied Microbiology Research

Downloads

Published

2026-03-31

Issue

Vol. 15 No. 1 (2026)

Section

Articles

License

Authors who publish with this journal agree to the following terms:

  1. Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a  Creative Commons Attribution-ShareAlike License  that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
  2. Authors are not restricted by the journal from entering into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., posting it to an institutional repository or publishing it in a book), with an acknowledgement of its initial publication in this journal.
  3. Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) after its publication in this journal. This approach aims to safeguard against potential plagiarism or unauthorized use of unpublished manuscripts, as outlined in the submission guidelines which state that this journal does not accept previously published manuscripts.