Eksplorasi Bakteri Selulolitik pada Tingkat Kematangan Gambut yang Berbeda di Kawasan Hutan Lindung Gunung Ambawang Kabupaten Kubu Raya

Abstract

Generally, peat soil has a low fertility rate. Based on the level of ripening, peat soil is composed of three categories, i.e. fibric, hemic and sapric. Biologically ripening peat soil is facilitated by cellulose degrading bacteria or cellulolytic bacteria. This research is aimed at determining the density and obtaining the cellulolytic bacteria genera on three levels of peat soil ripening. This research was carried out for three months, from April to June 2014. Soil samples were taken according to the level of ripening in the area of the Gunung Ambawang protected forest in the District of Kubu Raya. The isolation was carried out by using the Pour Plate method using the CMC (Carboxymethylcellulose) medium and bacteria density calculating using the TPC (Total Plate Count) method. Observation and characterization were made macroscopically, microscopically and through a series of biochemical tests. The result of research was obtained five bacteria genera, i.e. Neisseria, Actinobacillus,Acinetobacter,Staphylococcus and Micrococcus. Most cellulolytic bacteria genera were found in the hemic soil, whereas at least were on fibric soil. The highest rate of cellulolytic bacteria density in the research locations was found on the sapric soil, i.e. 1,845 x 10⁶ CFU/g, and the lowest on fibric soil, i.e. 7,8 x 10⁵ CFU/g and the density on hemic soil, i.e. 1,475 x 10⁶ CFU/g.