Uji Aktivitas Antibakteri Ekstrak Etanol Daun Kemangi (Ocimum sanctum L.) Terhadap Pertumbuhan Bakteri Escherichia coli dan Staphylococcus aureus

Authors

  • Maria Angelina, Masnur Turnip, Siti Khotimah Jurusan Biologi - Fakultas MIPA Universitas Tanjungpura Jl. Prof. Dr. H. Hadari Nawawi Pontianak - Kalimantan Barat

DOI:

https://doi.org/10.26418/protobiont.v4i1.9768

Keywords:

antibacterial, Basil leaves, Ocimum sanctum, Escherichia coli, Staphylococcus aureus

Abstract

Escherichia coli and Staphylococcus aureus are pathogenic bacteria that can cause intestinal and skin infections. One of the potential medicinal plants that is active against bacteria is basil leaves (Ocimum sanctum). This research aims to examine the antibacterial activity of ethanol extracts of basil leaves against
the growth of E. coli and S. aureus. The Research was conducted from September to December 2014. The ethanol extracts of basil leaves in this study used a concentration of 20%, 40%, 60%, 80%, 100%, and Thiamfenicol as a positive control. The antibacterial activity test to observe inhibition zone was done through the disc diffusion method. The results showed that the extracts of basil leaves were bacteriostatic. In this research, the highest inhibition zone occurred at a concentrations of 100%, while the optimum treatment in inhibiting the growth of E. coli and S. aureus was the concentration of 80%. Based on results of the phytochemical test, the ethanol extract of O. sanctum had a secondary metabolite compounds i.e. flavonoid, tannin, and essential oil that act as antibacterial. The Anova statistical results showed a significant difference in the diameters of the inhibition zone of the treatment of extracts with positive control.

Downloads

Published

2015-04-15

Issue

Vol. 4 No. 1 (2015)

Section

Articles

License

Authors who publish with this journal agree to the following terms:

  1. Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a  Creative Commons Attribution-ShareAlike License  that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
  2. Authors are not restricted by the journal from entering into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., posting it to an institutional repository or publishing it in a book), with an acknowledgement of its initial publication in this journal.
  3. Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) after its publication in this journal. This approach aims to safeguard against potential plagiarism or unauthorized use of unpublished manuscripts, as outlined in the submission guidelines which state that this journal does not accept previously published manuscripts.