SENYAWA GOLONGAN STEROID DARI DAUN ADAM HAWA (Rhoeo discolor (L,Her.) Hance) DAN UJI SUN PROTECTING FACTOR STEROID COMPOUND FROM ADAM HAWA (Rhoeo discolor (L,Her.) Hance) LEAVES AND SUN PROTECTING FACTOR TEST
DOI:
https://doi.org/10.26418/indonesian.v4i2.47674Abstract
The isolation of steroid compounds from Adam Hawa leaves has been carried out through maceration, partition, separation, and purification. The relatively pure BF2 isolate was 7 mg amorphous and with a melting point of 158-1700C. Results of 1H-NMR spectrum analysis with CDCl3 solvent, 500 MHz δH: 7,53 (d, J= 5,4 Hz, = C-H ); 7,72 (d, J= 5,7 Hz, = C-H); 5,35 (1H, d, J=5,15 Hz, =C-H); 3,51 (1H, m, Ar-CH), 0,93 (d, J= 6,2 Hz, 3H), 0,86 (3H, s), 0,84 (3H, s), 0,81 (3H, m), 0,84 (d, J= 5,2 Hz, 3H). The 1H-NMR spectrum of isolate BF2 similar to the 1H-NMR spectrum of ergosterol peroxide and ergosterol. So it is predicted that BF2 isolate has resembled structures with those compounds. In vitro sunscreen tests were performed on BF2 isolates and methanol extracts using a UV-Vis spectrometer. The SPF values of BF2 isolate and methanol extract at concentrations (ppm) of 120, 140, 160 and 180 respectively were 1.913; 1,684; 2252; 2483; and also 1,428; 1,655; 2,465; 1883. The results showed that BF2 isolate and methanol extract had weak activity as sunscreenReferences
Dalimartha S., (2003) Atlas Tumbuhan Obat Indonesia, Jakarta, Puspa Swara.
Gonzalez-Avila, M., Arriaga-Alba, M., De la Garza, M., PretelÃn, M. D. C. H. , DomÃnguez-Ortiz, M A, Fattel-Fazenda, S., and Villa-Treviro, S., (2003), Antigenotoxic, Anrimutagenic and ROS Scavenging Activities Of A Rhoeo Discolor Etanolic Crude Extract, Toxicologi In Vitro, 17(1): 77-83. https://doi.org/10.1016/s0887-2333(02)00120-0
Pratiwi R., Harlia, Wibowo M.A., (2017), Aktivitas Antiinflamasi dan Toksisitas Dari Ekstrak Daun Nanas Kerang (Rhoeo Discolor), Jurnal Kimia Khatulistiwa, 6(2):29-36.
Sitorus, R.M.H., Wullur, A., Yamlean, P., (2012), Isolasi dan Identifikasi Senyawa Flavanoid Pada Daun Adam Hawa (Rhoeo Discolor), Pharmacon, 1(1):53-57. https://doi.org/10.35799/pha.1.2012.446
Esviyani, V., Purwanti, L., dan Sadiyah, E.S., (2019) Potensi Antioksidan dan Tabir Surya Terhadap Daun Mawar (Rosa sp L.), Prosiding Farmasi, 5(2):170-176.
Putri, Y., Kartamihardja, H., & Lisna, I. (2019). Formulasi dan Evaluasi Losion Tabir Surya Ekstrak Daun Stevia ( Stevia rebaudiana Bertoni M ). Jurnal Sains Farmasi & Klinis, 6(1), 32–36
Schalka, S. and Reis V. M. S., (2011), Sun Protection Factor: Meaning and Controversies, Review An Bras Dermatol, 86(3):507-515.
Harborne, J.B., (1987) Metode Fitokimia: Penuntun Cara Modern Menganalisis Tumbuhan, Bandung, ITB.
Walters, C., Keeney, A., Wigal, C.T., Johnston, C.R. and Cornelius, R.D., (1997), Spectroscopy Analysis and Modelling of Sunscreens, J. Chem. Educ, 74(1):99. https://doi.org/10.1021/ed074p99
Dutra, E.A., Oliveira, D.A.G. da C., KedorHackmann, E.R.M. and Santoro, M.I.R.M., (2004) Determination of Sun Protection Factor (SPF) of Sunscreen by Ultraviolet Spectrophotometry, Journal od Pharmaceutical Sciences, 40(2):381-385.
Qian, Y., Qiu, X.Q., and Zhu, S.P., (2015), Lignin: A Nature-Inspired Sun Blocker For Broad-Spectrum Sunscreen, Green Chemistry, 17(1):320-324. https://doi.org/10.1039/c4gc01333f
Wang, Y., Xu, L., Ren, W., Zhao, D., Zhu, Y, and Wu, X., (2012), Bioactive Metabolites From Cheatomium Globosum L18, an Endophytic Fungus In The Medicinal Plant Curcuma Wenyujin, Phytomedicine, 19:364-368. https://10.1016/j.phymed.2011.10.011
Nowak, R., Drozd, M., Mendyk, E., Lemieszek, M., Krakowiak, O., Kisiel, O., Rzeski, W., and Szewczyk, K. (2016), A New Method For The Isolation Of Ergosterol And Peroxyergosterol As Active Compounds Of Hygrophoropsis aurantiaca and In Vitro Antiploliferative Activity Of Isolated Ergosterol Peroxide, Molecules, 21(946):1-10. https://10.3390/molecules21070946
Anonim, (2018), Ergosterol, www.fisherci.com, 23 Januari 2018.
Anonim, (2020) Ergosterol Peroxide, www.caymanchem.com, 9 November 2020.
Damogalad, V., Edy, H.E., dan Supriati, H.S., (2013), Formulasi Krim Tabir Surya Ekstrak Kulit Nanas (Ananas Comosus L Merr) Dan Uji In Vitro Nilai Sun Protecting Factor (SPF), Jurnal Ilmiah Farmasi-UNSRAT, 2(2):39-43. https://doi.org/10.35799/pha.2.2013.1577
Food and Drug Administration (FDA), (2003) Guidance for Industry Photosafety Testing, Pharmacology Toxycology Coordinating Committee in the Centre for Drug Evaluation and Research (CDER) at the FDA, USA.
Miller, T.F., (2003), US FDA Protocol for Determining Sun Protection Factor, in: Nadim A. Shaath, Sunscreens, Regulation and Commercial Development (3rd Edition), New York, Taylor dan Francis Group. https://doi.org/10.1201/b14170
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