EXPLORATION OF INDIGENOUS LEAD (PB)-RESISTANT BACTERIA FROM RUBBER WASTEWATER AS CANDIDATES FOR BIOREMEDIATION AGENTS

Authors

  • Emilya Putri Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Andalas https://orcid.org/0000-0002-5806-538X
  • Fuji Astuti Febria Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Andalas
  • Djong Hon Tjong

DOI:

https://doi.org/10.26418/jpmipa.v16i3.93437

Keywords:

Bacteria, Bioremediation, Heavy Metals, Resistant, Wastewater

Abstract

The presence of heavy metals such as lead (Pb) in rubber processing wastewater originates from coagulant materials, vulcanization processes, and raw water contamination. Pb is toxic and persistent, thus requiring proper treatment. This study aims to isolate indigenous bacteria from rubber wastewater that are resistant to Pb and have potential as bioremediation agents. Isolation was performed using the dilution-pour plate method, with selection on a medium containing Pb(NO₃)â‚‚. Resistance tests were conducted at increasing concentrations (100"“350 ppm). Three isolates (IS-1, IS-2, IS-3) were obtained, with IS-3 exhibiting the best growth (μ = 0.641 dayâ» ¹; generation time (G) 1.08 days). Molecular identification of the best bacterial isolate revealed that IS-3 is Pseudomonas protegens. This isolate shows potential as a bioremediation agent for Pb contamination.

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Published

2025-10-17

Issue

Vol. 16 No. 3 (2025): September 2025

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